ISBN: 978-1-83926-357-6
Year: 2022
Binding: HB
Price: US $ 280.00
Biotechnology is the use of biological processes, organisms, or systems to manufacture products intended to improve the quality of human life. Genetic engineering is the direct manipulation of an organism’s genome using biotechnology. It is a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms. New tools and products developed by biotechnologists are useful in research, agriculture, industry and the clinic. Modern biotechnology provides breakthrough products and technologies to combat debilitating and rare diseases, reduce our environmental footprint, feed the hungry, and use less and cleaner energy, and have safer, cleaner and more efficient industrial manufacturing processes. This book elucidates the concepts and innovative models around prospective developments with respect to biotechnology and genetic engineering. It talks in detail about the various methods and applications of this subject. It deals with drug targeting, gene therapy, genetic circuits, tissue engineering, growth hormones, transcriptome analysis, magnetic particles and genetically engineered bacteria. It examines the theories and practices related to genes and genetic modification. While understanding the long-term perspectives of the topics, the book makes an effort in highlighting their impact as a modern tool for the growth of the discipline. This book is an essential guide for both academicians and those who wish to pursue this discipline further.
Preface............................................................................................................ v
Chapter 1 Repurposing E. coli by Engineering Quorum Sensing and Redox
Genetic Circuits...........................................................................................1
Abstract...................................................................................................1
1. Introduction............................................................................................2
2. Optimizing protein expression: rational control of cell
condition.................................................................................................2
3. Decipher the bacterial dialog: quorum sensing..............................5
4. Bridging the bio-electro interface: Redox signaling and
electrogenetic systems........................................................................16
5. Conclusion............................................................................................20
Acknowledgements ...........................................................................21
Conflict of interest..............................................................................21
Chapter 2 Transcriptome Analysis and Genetic Engineering...........................30
Abstract.................................................................................................30
1. Introduction..........................................................................................31
2. Genetic engineering for human benefit..........................................33
3. GMOs and biosafety issues...............................................................34
4. Monitoring of GMOs..........................................................................34
5. Validation of genetically modified organisms...............................35
6. Transcriptome analysis for GMO validation.................................37
7. Conclusion............................................................................................39
Chapter 3 The Application of Nanodiamond in Biotechnology and
Tissue Engineering...................................................................................45
Abstract.................................................................................................45
1. Introduction..........................................................................................46
2. Diamond nanoparticles......................................................................50
3. Nanostructured diamond films........................................................57
4. Polymer-nanodiamond composites..................................................63
5. Conclusion............................................................................................65
Acknowledgements.............................................................................66
Chapter 4 Genetically Engineered Bacteria in Gene Therapy — Hopes and
Challenges...................................................................................................82
Abstract.................................................................................................82
1. Introduction..........................................................................................82
2. Bacteria as delivery vectors in gene therapy................................83
3. Type III delivery system: A promising strategy for targeting
intended cell tissues...........................................................................85
4. Issues to overcome for better translating the generated
proofs-of-concept to effective treatments in human....................87
5. Perspectives..........................................................................................88
Chapter 5 Genetic Engineering and Cloning: Focus on Animal
Biotechnology.............................................................................................95
1. Introduction..........................................................................................95
2. Genetic engineering: Timeline..........................................................98
3. Cutting and pasting the DNA........................................................100
4. DNA cloning......................................................................................103
5. Isolation, sequencing and synthesis DNA...................................103
6. Cloning vectors..................................................................................112
7. Practical application of genetic Engineering and cloning:
From transgenic animal models until cloning animal..............122
8. Future Prospects — Genetic engineering and cloning:
A dream or a nightmare?................................................................131
Acknowledgements .........................................................................134
Chapter 6 Magnetic Particles in Biotechnology: From Drug Targeting to
Tissue Engineering.................................................................................140
1. Introduction ......................................................................................140
2. Producing magnetic particles.........................................................141
3. Magnetic particles: Concerns towards in vivo use....................143
4. Applications of magnetic particles in the field of
biotechnology.....................................................................................146
5. Conclusion and perspectives..........................................................159
6. Acknowledgment..............................................................................160
Chapter 7 Genetic Engineering and Moral Responsibility ............................168
1. Introduction........................................................................................168
2. The rise of genetic science .............................................................170
3. Developments in genetic science and moral questions ...........172
4. Rights and responsibilities of science in society .......................193
5. Conclusions .......................................................................................195
Chapter 8 Genetic Engineering and Biotechnology of Growth Hormones ................................................................................................208
1. Introduction........................................................................................208
2. The growth hormone (GH) family................................................209
3. Growth hormone of animal origin ..............................................213
4. Biological potential of GHs ...........................................................215
5. Expression systems for growth hormones .................................217
6. Recombinant growth hormones.....................................................218
7. Technological platform for the production of recombinant
GHs .....................................................................................................219
8. Construction of GHs producing Pichia pastoris strains...........221
9. Production and analysis of recombinant hormones in the
flask......................................................................................................224
10. Production scaling in the bioreactor.............................................227
11. Semipurification of recombinant hormones.................................228
12. Testing the biological activity of recombinant hormones .......229
13. Contributions.....................................................................................230
14. Perspectives........................................................................................231
15. Acknowledgements...........................................................................232
Citation......................................................................................................239
Index...........................................................................................................241
