About Book

Chromatography has become a cornerstone of separation science, that branch of chemistry devoted to separating compounds from mixtures. Chromatography is one of several separation techniques defined as differential migration from a narrow initial zone. There are two main categories of chromatography: preparative and analytical. Analytical work uses small sample sizes; the objective is to separate compounds in order to identify then. Preparative work uses large quantities of samples and collects the output in bulk; the point of the chromatography here is to remove impurities from commercial product. The two are not mutually exclusive. Chromatography has numerous applications in biological and chemical fields. It is widely used in biochemical research for the separation and identification of chemical compounds of biological origin. This book elucidates the concepts and innovative models around prospective developments with respect to chromatography. It describes in detail the various basic concepts like gas chromatography, mass spectrometry, composites interaction, affinity chromatography, liquid chromatography, ion exchange chromatography, apocynaceae iridoids, and mathematical methods. This book is a valuable compilation of topics, ranging from the basic to the most complete theories in the field of chromatography. Such selected concepts that redefine this subject have been presented in this book. Different approaches, evaluations and methodologies on chromatography have been included. For someone with an interest and eye for detail, this book covers the most significant topics in this field.

Preface............................................................................................................v

Chapter 1 Introductory Chapter: Mathematical Methods in Liquid Chromatography - The State-of-the-Art Developments and Challenges.....................................................................................................1

1. Introduction............................................................................................1

2. Mathematical methods in liquid chromatography.........................2

3. Conclusion............................................................................................11

Chapter 2 Limit of Detection and Limit of Quantification Determination in Gas Chromatography...............................................................................17

1. Introduction..........................................................................................17

2. Limit of detection and limit of quantification..............................21

3. Conclusions..........................................................................................37

Nomenclature.......................................................................................40

Acknowledgements.............................................................................42

Chapter 3 A General Description of Apocynaceae Iridoids

Chromatography........................................................................................45

1. Introduction..........................................................................................45

2. Extracts preparation and iridoids isolated.....................................54

3. Chemical structures............................................................................58

4. Chromatographic separation.............................................................59

Chapter 4 Ion-Exchange Chromatography and Its Applications......................84

1. Introduction..........................................................................................84

2. Ion exchange chromatograpy applications..................................105

3. Conclusion..........................................................................................117

Chapter 5 Ion Exchange Chromatography - An Overview..............................122

1. Introduction........................................................................................122

2. Ion chromatography.........................................................................124

3. Instrumentation [6-9]........................................................................127

4. Advanced applications of ion chromatography..........................135

5. Conclusion..........................................................................................153

Chapter 6 Liquid Chromatography –Mass Spectrometry of Carbohydrates

Derivatized with Biotinamidocaproyl Hydrazide...........................156

1. Introduction........................................................................................156

2. Experimental .....................................................................................158

3. Results and discussion ...................................................................160

4. Conclusion .........................................................................................178

Acknowledgement ...........................................................................179

Chapter 7 Affinity Chromatography as a Tool for Quantification of Interactions

between Drug Molecules and their Protein Targets ....................186

1. Introduction........................................................................................186

2. Immobilization of target proteins on the surface of the stationary

phase ..................................................................................................188

3. Elution techniques ...........................................................................191

4. Examples of application .................................................................206

5. Conclusions .......................................................................................212

Chapter 8 Inverse Gas Chromatography in Characterization of Composites

Interaction.................................................................................................224

1. Introduction........................................................................................224

2. Theory of interaction ......................................................................225

3. Flory-Huggins parameters .............................................................226

4. Applications ......................................................................................234

5. Summary ...........................................................................................240

Chapter 9 A Method using Gas Chromatography – Mass Spectrometry for

the Detection of Mycotoxins from Trichothecene Groups A and B

in Grains...................................................................................................244

1. Introduction........................................................................................244

2. Experimental ............................................................................................. 250

3. Results and discussion ...................................................................254

4. Conclusions .......................................................................................263

Chapter 10 Major Volatile Compounds Analysis Produced from Mezcal

Fermentation Using Gas Chromatography Equipped Headspace

(GC–HS)....................................................................................................271

1. Introduction........................................................................................271

2. Mezcal process...................................................................................272

3. Volatile compounds produced in mezcal....................................274

4. Identification and quantification of volatile compounds in

mezcal fermentation by gas chromatography.............................278

5. Conclusion and perspectives..........................................................286

Citation......................................................................................................291

Index...........................................................................................................293

About Author

Chuck S Taylor received his Post Graduation in Applied Liquid Chromatography and Mass Spectrometry from Public University of Science, Perlis. He is a Guest Lecturer at several universities across Europe. He has wide-ranging areas of academic and research interests, which include mixture separation techniques, affinity chromatography, fast protein liquid chromatography and bonded phase. Taylor has contributed several articles and research papers in the diverse areas of chromatography. He is an active member of numerous scientific and technical societies.